Clare Reynolds, Haywards Heath, 11th June 2014
  • June 2014

Slide contents

  • Water testing for the 21st century
  • Overview
  • Key dates
  • Microbiology laboratory 1928
  • Culture method
  • Filters placed on filter support
  • Organisms removed from membrane filter manually, diluent containing organisms transferred to sterile bottle.
  • Known volume added to plate
  • 2 days
  • Suspect colonies counted and transferred to further agar plates for first stage of confirmatory tests.
  • Second stage confirmatory tests
  • TOTAL TIME FOR RELEASE OF RESULT
  • Limitations
  • Need something rapid, sensitive, specific, accurate and reliable
  • The PCR revolution 21st Century Microbiology
  • Does it work?
  • Current applications human infection – Singleplex assays
  • Current applications human infection – Multiplex assays
  • Genedisc Legionella PCR
  • Do we trust it?
  • PCR Limitations
  • Lee, J.V. et al. (2011). An international trial of quantitative PCR for monitoring Legionella in artificial water systems.
  • Suggested algorithm for interpretation of quantitative PCR results for routine monitoring of water from non-healthcare premises.
  • Legionella PCR – the laboratory experience
  • Case study/Pilot study
  • Case study 2
  • ENVIRONMENTAL MICROBIOLOGY UNIT THE TEAM
  • ENVIRONMENTAL MICROBIOLOGY UNIT LOGISTICS
  • ENVIRONMENTAL MICROBIOLOGY UNIT LOGISTICS
  • ENVIRONMENTAL MICROBIOLOGY UNIT GOVERNANCE, COMPLIANCE AND QUALITY
  • ENVIRONMENTAL MICROBIOLOGY UNIT RESULTS
  • ENVIRONMENTAL MICROBIOLOGY UNIT CONNECTIONS
  • ENVIRONMENTAL MICROBIOLOGY UNIT MORE THAN A RESULTS SERVICE
  • ENVIRONMENTAL MICROBIOLOGY UNIT CUSTOMER SERVICE
  • Plaudits