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Adam Stretton, Haywards Heath, 11th June 2014
June 2014
Slide contents
PCR & GeneDisc Overview
Summary
The Regulatory Environment
Micro-organism Testing
Viable But Non-Culturable (VBNC)
Rapid Microbiology Testing
Polymerase Chain Reaction (PCR)
The PCR Cycle - Denaturation
The PCR Cycle - Denaturation
The PCR Cycle - Annealing
The PCR Cycle - Elongation
The PCR Cycle - Repetition
Applications of PCR
Quantitative PCR (qPCR)
Quantitative PCR (qPCR) - signal
Quantifying the DNA
Quantifying the DNA
The advantages of PCR for Micro Testing
Pall’s Solution - GeneDisc®
The GeneDisc® Cycler
The GeneDisc® Plate
The GeneDisc® Cycler
GeneDisc® Software
GeneDisc® DNA Extraction
Technical Obstacles to PCR Adoption
Technical Obstacles to PCR Adoption
Technical Obstacles to PCR Adoption
The Future